Isobaric Labeling and Data Normalization without Requiring Protein Quantitation

Census 2: isobaric labeling data analysis

MOTIVATION We introduce Census 2, an update of a mass spectrometry data analysis tool for peptide/protein quantification. New features for analysis of isobaric labeling, such as Tandem Mass Tag (TMT) or Isobaric Tags for Relative and Absolute Quantification (iTRAQ), have been added in this version, including a reporter ion impurity correction, a reporter ion intensity threshold filter and an op...

cDNA Microarray Data Normalization

A hierarchical statistical modeling approach to analyze proteomic isobaric tag for relative and absolute quantitation data

MOTIVATION Isobaric tag for relative and absolute quantitation (iTRAQ) is a widely used method in quantitative proteomics. A robust data analysis strategy is required to determine protein quantification reliability, i.e. changes due to biological regulation rather than technical variation, so that proteins that are differentially expressed can be identified. METHODS Samples were created by mi...

MSnbase-an R/Bioconductor package for isobaric tagged mass spectrometry data visualization, processing and quantitation

UNLABELLED MSnbase is an R/Bioconductor package for the analysis of quantitative proteomics experiments that use isobaric tagging. It provides an exploratory data analysis framework for reproducible research, allowing raw data import, quality control, visualization, data processing and quantitation. MSnbase allows direct integration of quantitative proteomics data with additional facilities for...

SureLINK Chromophoric Biotin Labeling Kit for Labeling and Quantitation of Biotin in Protein Conjugates

SureLINK Chromophoric Biotin Labeling Kits allow for the easy labeling of proteins with biotin as well as an accurate, precise, and inexpensive method of quantitating biotin in biotin-protein conjugates. Accurate measurement of this parameter is critical for the efficient design of reproducible immunoassays. The basis of the quantitation method is a biotinylation reagent that contains a chromop...